Effect of Some Fungicides on Soil Biological Activities in Laboratory Conditions

Çiğdem KÜÇÜK, Derya YEŞİLORMAN, Cenap CEVHERİ
1.943 332

Abstract


The effects of mancozeb, carbendazim and tebuconazole on soil microbial populations, soil respiration, catalase and urease enzyme activities were determined through a 40 day incubation period in laboratory conditions. Soil sampling was carried out after 0, 5, 10, 15, 20, 25, 30, 35 and 40 days of incubation. Soil respiration showed fluctation with kind of the fungicide. Soil respiration, catalase and urease activity were significantly (p<0.05) affected from the fungicides. Enumeration of the soil microorganisms were made on different selective media. The mancozeb from fungicides used in this study increased the number of fungi at 20th day of incubation period. Carbendazim increased the number of actinomycetes at 20th day of incubation period and the number of total bacteria was increased at 40th day of incubation period. In this study, in the soils amended with mancozeb, tebucozole and carbendazim in 40th day of incubation period observed urease activity, but stimulation in the activity was recorded in 25th day of incubation period. It was concluded that fungicide applications affect soil microbial population, soil respiration, catalase activity and urease activity. 


Keywords


Fungicide, Soil Microflora, Catalase, Urease, Soil Respiration.

Full Text:

PDF (Türkçe)

References


Munoz-Leoz, B., Ruiz-Romera, E., Antigüedad, I., Garbisu, C., Tebuconazole application decreases soil microbial biomass and activity, Soil Biology and Biochemistry, 43, 2176-2183, 2011.

Tiryaki, O., Pestisid kullanımı ve Gıda Güvenliği, Gıda Güvenliği Dergisi, 1, 2-9, 2011.

Chen, S. K., Edwards, C. A., Subler, S., Effects of the fungicides benzomyl, captan and chlorothalonil on soil microbial activity and nitrogen dynamics in laboratory incubations, Soil Biology & Biochemistry, 33, 1971-1980, 2001.

Haktanır, K., Toprak biyolojisi, A.Ü. Ziraat Fak. Ders notları, Ankara, s. 85, 1991.

Martens, D. A., Bremner, J. M., Inhibitory effects of fungicides on hydrolysis of urea and nitrification of urea nitrogen in soil, Pesticide Science, 49, 344-352, 1997.

Wang, Y. S., Wen, C., Chiu, T., Yen, J. H., Effect of fungicide iprodione on soil bacterial community, Ecotoxicology and Environmental Safety, 59, 127-132, 2004.

Toros, S., Maden, S., Tarımsal savaşım yöntem ve ilaçları, A. Ü. Ziraat Fakültesi Yayınları, s. 332, Ankara, 1991.

Cycon, M., Piotrowska-Seğet, Z., Kozdroj, J., Responses of indigenous microorganisms to a fungicidal mixture of mancozeb and dimethomorph added to sandy soils, Int. Biodeterior. Biodegrad., 64, 316-323, 2010.

Lorbeer, J. W., Vincelli, P. C., Efficacy of dicarboximide fungicides and fungicide combinations for control of Botrytis leaf blight of onion in New York, Plant Disease, 74, 235-237, 1990.

Delen, N., Toros, N., Reduced sensitivity in Botrytis cinerea to thiram and mancozeb, XI th International Botrytis Symposium, 23-27 June, Wageningen, p. 31, 1996.

Tripathi, R. K., Vohra, K., Schkösser, E. Z., Effect of fungicides on the physiology of plants. III. Mechanism of cytokinin like antisenescent action of carbendazim on wheat leaves, Pflanzenler. Pflanzenschutz, 87, 631-639, 1980.

Ekundayo, E. O., Effect of common pesticides used in the Niger Delta basin of southern Nigeria on soil microbial populations, Environmental Monitoring and Assessment, 89, 35-41, 2003.

Yunlong, Y., Xiaaqang, H., Guohui, P., Yueqin, X., Hua, F., Effects of repeated applications of fungicide carbendazim on its persistence and microbial community in soil, J. Environmental Sci., 21, 179-185, 2009.

Dığrak, M., Kırbağ, S., Özçelik, S., Bazı pestisidlerin toprak mikroorganizmaları üzerine etkisi, Tr. J. Agriculture and Forestry, 20, 165-173, 1996.

Girvan, M. S., Campbell, C. D., Killham, K., Prossr, J. I., Glover, L. A., Bacterial diversty promotes community stability and functional resilience after perturbation, Environ. Microbiology, 7, 301-331, 2005.

Anderson, J. P. E., (Ed. A. L. Page) Soil respiration. In: methods of soil analysis, part 2, chemical and microbiological properties, ASA-SSSA, Madison, Winsconsin, 831-871, 1982.

Arcak, S., Qmar, S. M., Haktanır, K., Trifluralin’in toprakta nitrifkasyon ve katalaz aktivitesine etkileri, Tarım Bilimleri Dergisi, 1, 41-46, 1995.

Pepper, I. L., Gerba, C. P., Brendecke, J. W., Brendecke: Environmental Microbiology, A Laboratory Manual, Academic Press, New York, 1995.

Bending, G. D., Lincoln, S. D., Edmondson, R. N., Spatial variation in the degradation rate of the pesticides isoproturon, azoxystrobin and diflufenican in soil and its relationship with chemical and microbial properties, Environmental Pollution, 139, 279-287, 2006.

Domsch, K. H., Jabnow, G., Anderson, T. H., An ecological concept for the assessment of side effects of agrochemicals on soil microorganisms, Research Review, 86, 65-105, 1983.

Yan, H., Wang, D., Dang, B., Tang, F., Wang, B., Fang, H., Yu, Y., Dissipation of carbendazim and chloramphenical alone and in combination and their effects on soil fungal: bakterial rations and soil enzyme activities, Chemosphere, 84, 634-641, 2011.

Cernohlavkova, J., Jarkovskly, J., Hofman, J., Effects of fungicides mancozeb and dinocap on carbon and nitrogen mineralization in soils, Ecotoxicology and Environmental Safety, 72, 80-85, 2009.

Strickland, T. C., Potter T.I., Joo H., Tebuconazole dissipation and metabolism in Tifton loamy sand during laboratory incubation, Pest Mange. Sci., 60, 703-709, 2004.

Rasool, N., Reshi, Z. A., Effect of fungicide Mancozeb at different application rates on enzyme activities in a silt loam soil of the kashmir Himalaya, India, Tropical Ecology, 51, 199-205, 2010.

Moreno, J. L., Aliaga, A., Navarro, S., Hernandez, T., Garcia, C., Effects of atrazine on microbial activity in semiarid soil, Applied Soil Ecology, 35, 120-127, 2007.

Tabatabai, M., Effect of trace elements on urease activity in soils, Soil Biology and Biochemistry, 9, 9-13, 1997.

Rahman, M. M., Kim, T., Rhee, I., Kim, J., Effect of the fungicide chlorothalonil on microbial activity and nitrogen dynamics in soil ecosystem, Agric. Chem. Biotechol., 46, 169-173, 2003.

Pazo, C., Rodales, V., Salmeron, M. V., Martinez-Toledo, G., Vele, R., Effects of fungicides maneb and Mancozeb on soil microbial populations, Toxicological and Environmental Chemistry, 43, 123-132, 1994.

Köller, W., Chemical approaches to monaging plant pathogens. In: Ruberson, J. R. ed., Handbook of Pest Management Marcel Dekker, pp. 337-376, New York, 1999.

Magarey, R. C., Bull, J. I., Effect of the dithiocarbamate fungicide mancozeb on sugarcane growth and soil biology in yield decline affected soils, Proc. Aust. Soc. Sugar Cane Technol., p. 25, 2003.