Physio-Biochemical and Molecular Responses in Transgenic Cotton under Drought Stress

Muhammad Bilal SARWAR, Sajjad SADIQUE, Sameera HASSAN, Sania RIAZ, Bushra RASHID, Bahaeldeen Babiker MOHAMED, Tayyab HUSNAIN
301 58

Öz


Drought decreases the growth and productivity in cotton. Heat shock proteins accumulate in plants under water stress
to protect the biochemical and physiological processes at the molecular level. In this study, plants of T2 segregating
generation of transgenic cotton, containing small heat shock protein gene (GHSP26) was compared with wild type
plants for biochemical, physiological and molecular responses under different periods of drought stress. Transgenic
plants accumulated 30% higher proline content than the wild type. Lipid peroxidation activity was reduced in transgenic
plants which showed that the drought tolerance efficiency has been improved. Leaf relative water content was 69%
and 45% in transgenic and wild-type plants, respectively at 10-day drought stress. Similarly, transgenic plants showed
better performance for photosynthesis, stomatal conductance, transpiration and osmotic potential as compared to wild
type. Real-time quantitative PCR of GHSP26 and some other drought responsive genes such as Gh-POD, Gh-RuBisCO,
Gh-LHCP PSII, Gh-PIP, Gh-TPS and Gh-LEA have supported the higher expression and proved drought tolerance in
transgenic plants. The overexpression of GHSP26 in transgenic plants improved the biochemical such as proline content
and lipid peroxidation activity and physiological parameters like photosynthesis, osmotic potential and water related
attributes. Hence, this study may be extended for selection of homozygous lines and breeding to improve the drought
tolerance activity in plants.


Anahtar kelimeler


Gene expression; Gossypium hirsutum; Physiological analysis; Genetically modified cotton; Water stress; Biochemical analysis

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