Gamma rays and EMS induced Flower Color Mutation in Grasspea (Lathyrus sativus Linn.)

Pegah Ramezani, Morteza Siavoshi, Anil D More, Mahboubeh Ebrahimi


To induce flower color mutants in Grasspea (Lathyrus sativus Linn.) variety “Pusa-24“, the fresh seeds were exposed to 4 different doses of gamma radiation as physical mutagens (100, 200, 300 and 400 Gy) and treated with 4 different concentration of Ethyl Methanesulphonate as chemical mutagen (5, 10, 15 and 20 mM ) and also combination of both mutagens. Due to mutagenesis broad range of flower color mutants in M2, M3 and M4 were obtained. Various unique and different flower color mutations like pink, purple, white and yellow detected against blue which is natural color in control progenies. Maximum flower color frequency was recorded in EMS treated seeds (20 mM) in 4th generation and lowest mutation frequency in 2nd generation was obtained in gamma rays (100 Gy) treated seeds. The results showed more flower color mutations happened in more dose /concentration of mutagens in every generation

Anahtar kelimeler

Flower color, Mutation, Grasspea, Gamma radiation, EMS