Comparison of different bioassay methods for determining insecticide resistance in European Grapevine Moth, Lobesia botrana (Denis & Schiffermüller) (Lepidoptera: Tortricidae)

Enver Durmuşoğlu, Ahmet Hatipoğlu, M. Oktay Gürkan, Graham Moores
2.135 441


The European Grapevine Moth [Lobesia botrana (Denis & Schiffermüller) (Lepidoptera: Tortricidae)] is a major pest in vineyards and insecticides are heavily used in its management. Currently, there is no IRAC approved bioassay method for L. botrana, so this study aimed to develop a suitable susceptibility test. For this purpose, 10 different populations were cultured from Manisa (Turkey) that province an important place in the world of viticulture. Diet-incorporation, topical application and diet covering methods selected as potential methods. Three different bioassay techniques (diet-incorporation, diet covering and topical application) were compared using indoxacarb, deltamethrin, spinosad and chlorpyrifos-ethyl on three different populations. Results revealed that the diet-incorporation by mixing was the most appropriate method for testing insecticide resistance against the European Grapevine Moth.


Bioassay, European grape moth, insecticide resistance, diet incorporation, diet covering, topical application

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