Comparison of different bioassay methods for determining insecticide resistance in European Grapevine Moth, Lobesia botrana (Denis & Schiffermüller) (Lepidoptera: Tortricidae)

Enver Durmuşoğlu, Ahmet Hatipoğlu, M. Oktay Gürkan, Graham Moores
2.039 429

Abstract


The European Grapevine Moth [Lobesia botrana (Denis & Schiffermüller) (Lepidoptera: Tortricidae)] is a major pest in vineyards and insecticides are heavily used in its management. Currently, there is no IRAC approved bioassay method for L. botrana, so this study aimed to develop a suitable susceptibility test. For this purpose, 10 different populations were cultured from Manisa (Turkey) that province an important place in the world of viticulture. Diet-incorporation, topical application and diet covering methods selected as potential methods. Three different bioassay techniques (diet-incorporation, diet covering and topical application) were compared using indoxacarb, deltamethrin, spinosad and chlorpyrifos-ethyl on three different populations. Results revealed that the diet-incorporation by mixing was the most appropriate method for testing insecticide resistance against the European Grapevine Moth.


Keywords


Bioassay, European grape moth, insecticide resistance, diet incorporation, diet covering, topical application

Full Text:

PDF


DOI: http://dx.doi.org/10.16970/ted.93098

References


Brown, T.M., 1981, Counter measures for insecticide resistance. Bulletin of the Entomological Society of America, 27:198-202.

Civolani, S., M. Boselli, A. Butturini, M. Chicca, E.A. Fano & S. Cassanelli, 2014. Assessment of insecticide resistance of Lobesia botrana (Lepidoptera: Tortricidae) in Emilia-Romagna Region. Journal of Economic Entomology, 107:1245- 1249.

Delen, N., C. Koplay, M. Yıldız, N. Güngör, P. Kınay, F. Yıldız & A. Coşkuntuna, 2004. Sensivity in Botyrtis cinerea isolates to some fungicides with specific mode of action. XIII. Botyrtis Symposium (25-31 October 2004, Antalya), Abstracts, 131p.

Ffrench-Constant, R.H. & R.T. Roush, 1990. “Resistance Detection and Documentation: The Relative Roles of Pesticidal and Biochemical Assays, 4-38”. In: Pesticide Resistance in Arthropods (Eds: R.T. Roush & B.E. Tabashnik), Chapman and Hall, New York.

Gunning, R.V., 1993. Comparison of the two bio-assay techniques for larvae of Helicoverpa sp. (Lepidoptera: Noctuidae). Journal of Economic Entomology, 86: 234-238.

Hatipoglu A., E. Durmuşoğlu & M.O. Gürkan, 2015. Manisa ili bağ alanlarında Salkım güvesi [Lobesia botrana (Denis & Schiffermüller) (Lepidoptera: Tortricidae)] popülasyonlarının insektisit direncinin belirlenmesi, Turkish Journal of Entomology, 39 (1): 55-65.

IRAC, 2014. Resistance Definition, Backround, Development. (Web page:http://www.irac-online.org/about/resistance) (Date accessed: Feb 2014).

Perez, C.J., J.D. Tang & A.M. Shelton, 1997. Comparison of leaf-dip and diet bioassays for monitoring Bacillus thuringiensis resistance in field populations of diamondback moth (Lepidoptera: Plutellidae), insecticide resistance and resistance management. Journal of Economic Entomology, 90: 94-101.

Rapagnani, M. R., V. Cafferelli, M. Barlettoni & F. Mineli, 1990. Descrizione di un alleuamento in labratorio, dele tignolotta dell’ura Lobesia botrana Den.-Schiff. (Lepidoptera: Tortricidae) su on nuova alimento semi-sintetica, Bolletino dell Istituto di Entomologia ‘Guido Grandi’ dell Universito di Bologna, XLIV: 57-64.

Roush, R.T. & B.E. Tabashnik, 1990. Pesticide Resistance in Arthropods. Chapman and Hall, New York and London, 303p.