Effect of collection time on Chemical composition and Antibacterial activity of Flower Essential oil of Ocimum canum (sims) grown in Nigeria.

Lamidi Ajao Usman, Abdulrauf Olaniyi Yussuf, Bola Kudirat Saliu, Bolatito Eunice Olanipekun, Nusirat Elelu
1.270 204


Flowers (1000g) of Ocimum canum harvested at different time (7am, 10am, 1pm, 4pm and 7pm) in a day were separately hydrodistilled and yielded 0.19 - 0.27% (w/w) of essential oils.  GC, GC-MS analyses revealed that the oils were predominated by oxygenated monoterpenoids (51.2-74.4%). Hydrocarbon monoterpenoids constituted 6.2-10.2% of the oils. Percentage composition of hydrocarbon and oxygenated sesquiterpenoids in the oils were in the range of 1.3-22.0%. The most abundant constituent of the oils was linalool (40.5-58.7%). Other principal constituents were; limonene (0.6 –7.5%), terpinen-4-ol (1.4–5.6%), eugenol (4.4–8.9%), geranyl acetate (0.2–4.9%), α-trans-bergamotene (3.2–9.4%) and E-isoeugenol (4.1–5.5%). The predominance of linalool in the oils showed that the oils were of linalool chemotype. Antibacterial activity of the oils was evaluated against Staphylococcus aureus and Escherichia coli using agar diffusion method. Irrespective of the time of collection of the flower, the oils were found to be active against the tested organisms. However, they are more active on Escherichia coli than Staphylococcus aureus. The activity of the oils on the organisms was concentration dependent.


Ocimum canum, Chemotype, linalool, terpene synthase, antibacterial.

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Iwu MM, 1993. Handbook of African medicinal plants. CRC Press, New York, pp: 164-166.

Ngassoum MB, Ousmaila H, Ngamo LT, Maponmetsem PM, Jirovetz L, Buchbauer G. Aroma compounds of essential oils of two varieties of the spice plant Ocimum canum Sims. From northern Cameroon. J. Food Comp. Anal., 2004; 17: 197–204.

Bauer K, Garbe D, Surburg H. Common Fragrance and Flavour Materials, 2 edn., VCH Verlagsgesellschaft mbH, Weinheim. 1990.

Fun CE, Baerheim-Svendsen A. Flavour Frag. J. 1990; 5: 173—177.

Tamil Selvi M, Thirugnanasampandan R, Sundarammal S. Antioxidant and cytotoxic activities of essential oil of Ocimum canum Sims. from India. Journal of Saudi Chemical Society. 2015; 19: 97–100.

Bhattacharya A, Ashok A, Navnota S, Jagbeer C. Evaluation of some anti-oxidative constituents of three species of Ocimum. International Journal of Life Science. 2014; 8(5): 14-17.

Pradyut B. Evaluation of antibacterial activities of leaf extracts of two medicinal plants Ocimum canum Sims and Ocimum tenuiflorum Linn., J. Microbiol. Biotech. Res., 2013; 3 (3):20-23.

Behera S, Babu SM, Ramani YR, Choudhury PK, Panigrahi R. Phytochemical Investigation And Study On Antioxidant Properties Of Ocimum Canum Hydro-Alcoholic Leaf Extracts. Journal of Drug Delivery & Therapeutics. 2012: 2(4), 122-128.

Ntezurubanza L, Scheffer JJC, Looiqlan A. Composition of the essential oil of Ocimum canum grown in Rwanda. Pharmaceutisch Weekblad Scientific Edition. 1985; 273, Vol. 7.

Ekundayo O, Laakso I, Hiltunen R. Constituents of the Volatile Oil from Leaves of Ocimum canum Sims. Flavour and fragrance journal. 1989; 4.17-18.

Martins AP, LIgia R, Salgueirol R, Felix T, Salvador C, Joseph C, Antonio PC, Tomás A. Composition of the Essential Oils of Ocimum canum, 0. gratissimum and 0. Minimum, Planta Med., 1999; 65

Jeferson C, Nascimento L, Barbosa CA, Vanderlucia FP, Jorge MD, Renato F, Luiz AMS, Robson SA. Chemical composition and antimicrobial activity of essential oils of Ocimum canum Sims. and Ocimum selloi Benth. Annals of the Brazilian Academy of Sciences. 2010; 83(3): 787-799.

Philippe B, Ntonga PA, Eric- Moïse BF, Gisèle AFD, Joseph LT. Chemical composition and residue activities of Ocimum canum Sims and Ocimum basilicum L essential oils on adult female Anopheles funestus ss, Journal of Animal &Plant Sciences. 2013; 19(1): 2854-2863.

Ntonga PA, Nicolas B, Elisabeth M, Lengo M, Philippe B, Philippe G. Activity of Ocimum basilicum, Ocimum canum, and Cymbopogon citratus essential oils against Plasmodium falciparum and mature-stage larvae of Anopheles funestus s.s. EDP Sciences Parasite. 2014; 21: 33

Bassole IHN, Nebie R, Savadogo A, Ouattara CT, Barro N, Traore SA. Composition and antimicrobial activities of the leaf and flower essential oils of Lippia chevalieri and Ocimum canum from Burkina Faso. African Journal of Biotechnology. 2005; 4 (10): 1156-1160.

Usman LA, Watti OI, Ismaeel RO, Ojumoola AO. Effect of drying on yield, chemical composition and Insecticidal activity of leaf essential oil of sweet orange (Citrus sinensis). Journal of the Turkish Chemical Society (JOTCS). 2016; 3(1):1-18.

Saliu BK, Agbabiaka TO, Sule IO, Gambari-Ambali RO. Assessment of Local Methods of Processing for the Preservation of the Physico-Chemical Properties and Microbiological Quality of two Local Cheeses in Ilorin, Nigeria. Journal of Microbiology Biotechnology and Food Science. 2014; 3 (4) 337-342.

British Pharmacopoeia, London, H.M. Stationary. 1980; 11: 109.

Jennings W, Shibamoto T. Qualitative Analysis of Flavour volatiles by Gas Chromatography. Academic Press, 1980; New York.

Adams RP. Identification of Essential Oil components by Gas Chromatography and Mass Spectrometry. 4th ed. Allured Publ. Corp.Carol Stream. IL. USA. 2012; ISBN: 978-1-932633-21-4.

Joulain D, Koenig WA. The Atlas of Spectra data of sesquiterpene hydrocarbons. 1998; E.B. Verlog Hamburg, Germany.

Sartoratto A, Machado ALM, Delarmelina C, Figueira GM, Duartssential MCT. Oils from Aromatic Plants used in Brazil. Brazilian J. Microbiology. 2004; 35: 275-280.

Degenhardt J, Kollner TG, Gershenzon J. Monoterpene and sesquiterpene synthases and the origin of terpene skeletal diversity in plants. Phytochem. 2009; 70: 1621–1637. DOI: 10.1016/j.phytochem.2009.07.030.

J. Turk. Chem. Soc., Sect. A: Chem.