Low-copy nuclear gene and McGISH resolves polyploid history of Eleusine coracana and morphological character evolution in Eleusine

Qing LIU, Bin JIANG, Jun WEN, Paul Michael PETERSON
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Abstract


Eleusine coracana (L.) Gaertn. (finger millet) is the third most important cereal crop in semiarid regions of the world, but the degree of relatedness of finger millet with other species in the genus is unverified. The study of morphological character evolution in Eleusine Gaertn. has lagged behind due to lack of relevant research. Polyploidy history of finger millet was analyzed using waxy sequences together with multicolor genomic in situ hybridization (McGISH). In the waxy phylogenetic tree, sequences of 2 homoeologous loci were isolated from 3 tetraploids: Eleusine coracana, E. africana Kenn.-O'Byrne, and E. kigeziensis S.M.Phillips. The 3 species grouped with E. indica (L.) Gaertn. and E. tristachya (Lam.) Lam. in the W-A1 clade (A-type sequences). The W-B1 and W-B2 clades (B-type sequences) were composed of the E. africana-E. coracana and the E. jaegeri Pilg.-E. kigeziensis subclades, respectively. Eleusine indica probes produced stronger signals of relative intensities across A-genome chromosomes of finger millet than did the E. tristachya probes. The waxy phylogenetic tree and McGISH evidence support E. indica and E. tristachya (or its extinct sister or ancestor) as the primary and secondary A-genome parents for finger millet, respectively. The most likely scenario is that the B-genome donor is extinct. Five morphological characters were found to be homoplasious by optimization on waxy gene tree.

Keywords


Key words: Genome parents, multicolor genomic in situ hybridization, phylogenetic analysis

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References


Avdulov NP (1931). Karyo-systematische Untersuchung der Familie Gramineen. Bull Appl Bot Genet Plant Breeding 44: 14–28 (in Russian with a summary in English).

Barbeau WE, Hilu KW (1993). Protein, calcium, iron, and amino acid content of selected wild and domesticated cultivars of finger millet. Plant Food Hum Nutr 43: 97–104.

Bisht MS, Mukai Y (2001). Genomic in situ hybridization identifies genome donor of finger millet (Eleusine coracana). Theor Appl Genet 102: 825–832.

Bisht MS, Mukai Y (2002). Genome organization and polyploid evolution in the genus Eleusine (Poaceae). Plant Syst Evol 233: 243–258.

Bradley RD, Hillis DM (1997). Recombinant DNA sequences generated by PCR amplification. Mol Biol Evol 14: 592–593.

Brassac J, Jakob SS, Blattner FR (2012). Progenitor-derivative relationships of Hordeum polyploids (Poaceae, Triticeae) inferred from sequences of TOPO6, a nuclear low-copy gene region. PLoS ONE 7: e33808.

Chennaveeraiah MS, Hiremath SC (1973). Genome relationship of Eleusine tristachya and E. floccifolia. J Cytol Genet 8: 1–5.

Chester M, Gallagher JP, Symonds V, Da Silva AVC, Mavrodiev EV, Leitch AR, Soltis PS, Soltis DE (2012). Extensive chromosomal variation in a recently formed natural allopolyploid species, Tragopogon miscellus (Asteraceae). P Natl Acad Sci USA 109: 1176–1181.

Darling E, Samis KE, Eckert CG (2008). Increased seed dispersal potential towards geographic range limits in a Pacific coast dune plant. New Phytol 178: 424–435.

Dechyeva D, Gindullis F, Schmidt T (2003). Divergence of satellite DNA and interspersion of dispersed repeats in the genome of the wild beet Beta procumbens. Chromosome Res 11: 3–21.

Drea S, Leader DJ, Arnold BC, Shaw P, Dolan L, Doonan JH (2005). Systematic spatial analysis of gene expression during wheat caryopsis development. Plant Cell 17: 2172–2185.

Flavell RB (1986). Repetitive DNA and chromosome evolution in plants. Philos T Roy Soc B 312: 227–242.

Fortune PM, Schierenbeck KA, Ainouche AK, Jacquemin J, Wendel JF, Ainouche ML (2007). Evolutionary dynamics of waxy and the origin of hexaploid Spartina species (Poaceae). Mol Phylogenet Evol 43: 1040–1055.

Hanski I, Eralahti C, Kankare M, Ovaskainen O, Siren H (2004). Variation in migration propensity among individuals maintained by landscape structure. Ecol Lett 7: 958–966.

Hilu KW (1980). Eleusine tristachya (Lam.) Lam. Madroňo 27: 177– 1

Hilu KW (1988). Identification of the “A” genome of finger millet using chloroplast DNA. Genetics 118: 163–167.

Hilu KW (2003). Eleusine. In: Barkworth ME, Capels KM, Long S, Piep MB, editors. Flora of North America North of Mexico, Vol. New York, NY, USA: Oxford University Press, pp. 109–110. 11 Wallace RS, Jansen RK (1995). DNA evidence for multiple origins of intergeneric allopolyploids in annual Microseris (Asteraceae). Plant Syst Evol 198: 253–265.

Wang SJ, Guo BZ, Li JH (1986). The major types of caryopses of the Chinese Gramineae in relation to systematics. Acta Phytotax Sin 24: 327–345 (in Chinese with an abstract in English).

Watson L, Dallwitz MJ (1992). Grass Genera of the World. Wallingford, UK: CAB International.

Werth CR, Hilu KW, Langner CA (1994). Isozyme of Eleusine (Gramineae) and the origin of finger millet. Am J Bot 81: 1186–1197.