Influence of blood storage time on viral RNA extraction for the detection of bovine viral diarrhea virus in persistently infected cattle

Yingxia LIU, Jie CAO, Junjie ZHANG, Kai HUANG, Changming QI
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Abstract


This study aimed to determine the maximum permissible storage times of blood and serum infected with bovine viral diarrhea virus (BVDV). Viral RNA was successfully extracted from blood and serum that were stored at room temperature (RT) for 7 days and was detected by 1-step RT-PCR. The results of this study demonstrate that BVDV-infected blood can be stored at RT for 7 days and that serum can be stored for 10 days without influencing the viral RNA extraction for the detection of BVDV.

Keywords


Key words: Bovine viral diarrhea virus, viral RNA extraction, storage time, blood, serum

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References


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