Effect of Astragalus and Astragalus Polysaccharide on Antimicrobial Peptides PR-39 and Protegrin-1 Gene Expression in Pigs

Youjin TU, Chunlan XU, Yizhen WANG, Guojia YAO, Tizhong SHAN
379 68

Abstract


This experiment was conducted to study the effects of micron Astragalus, 80 mesh Astragalus and Astragalus polysaccharide (APS) on antimicrobial peptides PR-39 and protegrin-1 gene expression in pigs using semi-quantitative RT-PCR analysis. One hundred twenty 60-day-old Duroc x Landrace x Yorkshire piglets (21.88 ± 1.26 kg of average initial body weight) were randomly allotted to 4 treatments. Each treatment had 3 replicates (i.e. pens) with 10 pigs per pen. The basal diets were supplemented with 0, 5 g kg-1 APS, 5 g kg-1 80 mesh Astragalus, and 5 g kg-1 micron Astragalus, respectively, and the feeding experiment lasted 30 days. After the feeding experiment, 12 pigs with 3 animals in each treatment were chosen to determine the effect of Astragalus or APS on PR-39 and protegrin-1 mRNA expression of pigs. The results showed that supplementation with 5 g kg-1 micron Astragalus significantly (P < 0.05) enhanced antimicrobial peptides PR-39 and protegrin-1 gene expression. Pigs fed the diet containing 5 g kg-1 80 mesh Astragalus also had higher PR-39 and protegrin-1 gene expression level than did the control (P < 0.05). However, micron Astragalus was more effective than 80 mesh Astragalus in enhancing PR-39 and protegrin-1 gene expression. Supplementation with 5 g kg-1 APS reduced PR-39 and protegrin-1 gene expression (P < 0.05).

Keywords


Astragalus, PR-39, protegrin-1, antimicrobial peptide, pigs

Full Text:

PDF