miR482 ve Bitkilerdeki İzoformları

Abdil Hakan EREN, Emre İLHAN, Behcet İNAL
1.036 265

Öz


Bitkilerdeki miR482 aile üyeleri genelde 22 nükleotid uzunluğunda diğer mikroRNA (miRNA) ailelerinden daha değişken ve sıra dışı dizilere sahiptir. Çalışmalar miR482’nin hastalık direnciyle ilişkili nükleotit-bağlayıcı lösince zengin tekrarlı (Nucleotide binding-site leucine-rich repeat, NB-LRR) genlerle ilgili olduğunu göstermektedir. Bitki genomlarında kodlanmış çeşitli NB-LRR’ler birçok patojen tanımayı sağlayan bir çeşit bağışıklık sistemidir. NB-LRR proteinleri normal patojenlere karşı efektör-tetikleme bağışıklıklığı ile ilişkilidir. Bitkide temelden gelen bağışıklık reseptörleri tanılayıcı reseptör yapıları (Pattern recoginition receptor, PRR) ve direnç (Resistance, R) proteinleridir. R genlerinin çoğu NB-LRR etki ile hücrede bağışıklık proteinlerini kodlarlar. miR482, miR1448, slmiR2118 ve ath-miR472 hastalık direnci ile ilişkili miRNA’lardır. Yapılan bazı çalışmalarda ise miR482’nin miR1448 homoloğu olduğu bildirilmiş ve filogenetik analizler miR482’nin tandem duplikasyon ürünü miR1448 olabileceğini göstermektedir. Evrimsel süreçte bu miRNA’lar aynı transkripti hedeflemişlerdir. miR482 izoformlarının baskılanması bitkiyi patojene karşı hassaslaştırırken miR482’nin soyada nodülasyon oluşumunda ve mikorizaların çalışma prensibinde etkili olabileceği düşünülmektedir. Giderek artan kanıtlar miRNA482’nın patojen saldırısında hastalık direnci tepkilerinde kritik roller oynadığını göstermektedir.


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DOI: http://dx.doi.org/10.19159/tutad.48747

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