The Effect of Dielectric Barrier Discharge Plasma Treatment on the Microorganisms Found in Raw Cow’s Milk
Milk is an essential source of nutrition especially for the breastfed infants. Sterilization of milk is necessary because it can be contaminated by microorganisms due to unhygienic collection and storage conditions. In this study, the sterilization of raw cow milk was performed by using dielectric barrier discharge (DBD) plasma method. Raw milk was transferred to the plasma reactor and dielectric barrier discharge cold plasma was performed by changing various parameters including voltage, exposure time and frequency. It was found that dielectric barrier discharge cold plasma is very effective at sterilization of raw cow milk particularly at room temperature. The optimum parameters that wee demonstrated to completely kill the bacteria in raw milk were experimentally determined to be a 3 kV application voltage, 3 min exposure time and 500 Hz frequency. Additionally, there was almost no important change in pH value of cow milk after DBD plasma treatment (The average pH was 6.2). Pathogen microorganisms found in milk produces metabolites (during storage and transport) that have adverse effects on health. The method developed by us in this study will be used in a future study to develop a prototype of a sterilization device that can be integrated into the current milking system and can be continuously applied. Thus, the sterilization of milk during milking process could potentially be an extremely effective method for maintaining its quality and nutritional value. Furthermore, since the DBD plasma method is an ultra fast process that operates under ambient temperatures (ideal for thermolabile products) at a low running cost and is environment-friendly, it can be used for the sterilization of a wide range of liquid food products.
Tam metin:PDF (English)
Akishev, Y., Grushin, M., Karalnik, V., Trushkin, N., Kholodenko, V., Chugunov, V., Kobzev, E., Zhirkova, N., Irkhina, I., Kireev, G., 2008. Atmospheric-pressure, nonthermal plasma sterilization of microorganisms in liquids and on surfaces. Pure and Applied Chemistry, 80(9): 1953-1969.
Azevêdo, M.C., Santos, G.B., Zocche, F., Horta, M.C., Dias, F.S., Costa, M.M., 2014. Microbiological evaluation of raw milk and coalho cheese commercialised in the Semi-Arid Region of Pernambuco, Brazil. African Journal of Microbiological Research, 8(3): 222-229.
Bali, O.S., Lajnef, R., Felfoul, I., Attia, H., Ayadi, M.A., 2013. Detection of Escherichia coli in unpasteurized raw milk. International Journal of Agricultural and Food Science, 3(2): 53-55.
Banu, S.M., Sasikala, P., Aruna, D., Kavitha, V., Yazhini, G., Lavanya, R., 2012. Cold plasma as a novel food processing technology. International Journal of Emerging Trends in Engineering and Development, 4(2): 803-818.
Barbosa-Canovas, G.V., Pothakamury, K.R., Palou, E., Swanson, B.G., 1997. Nonthermal Preservation of Foods. 2nd ed., Marcel Dekker Incorporation, New York.
De Schweinitz, E.A., 1895. The Pasteurization and Sterilization of Milk, Yearbook of The United States Department of Agriculture, Washington DC., pp 331-356.
Duan, Y., Huang, C., Yu, Q., 2005. Low-temperature direct current glow discharges at atmospheric pressure. IEEE Transactions on Plasma Science, 33(2): 328-329.
Kim, H.J., Yong, H.I., Park, S., Kim, K., Choe W., Jo C., 2015. Microbial safety and quality attributes of milk following treatment with atmospheric pressure encapsulated dielectric barrier discharge plasma. Food Control, 47: 451-456.
Kogelschatz, U., 2007. Twenty years of Hakone symposia: From basic plasma chemistry to billion dollar markets. Plasma Processes and Polymers, 4(7-8): 678-681.
Korachi, M., Turan, Z., Şentürk, K., Şahin, F., Aslan, N., 2009. An investigation into the biocidal effect of high voltage AC/DC atmospheric corona discharges on bacteria, Yeasts, Fungi and Algae. Journal of Electrostatics, 67(4): 678-685.
Laroussi, M., 2005. Low temperature plasma-based sterilization: Overview and State-of-Art. Plasma Processes and Polymers, 2(5): 391-400.
Miau, H., Yun, G., 2012. The effect of air plasma on sterilization of Escherichia coli in dielectric barrier discharge. Plasma Science and Technology, 14(8): 735-740.
Misra, N.N., Tiwari, B.K., Rahavarao, K.S.M.S., Cullen, P.J., 2011. Nonthermal plasma inactivation of food-borne pathogens. Food Engineering Reviews, 3(3-4): 159-170.
Montie, T.C., Kelly-Wintenberg, K., Roth, J.R., 2000. An overview of research using the one atmosphere uniform glow discharge plasma (OAUGDP) for sterilization of surfaces and materials. IEEE Transactions on Plasma Science, 28(1): 41-50.
Morent, R., De Geyter, N., Verschuren, J., De Clerck, K., Kiekens, P., Leys, C., 2008. Non-thermal plasma treatment of textiles. Surface and Coatings Technology, 202(14): 3427-3449.
Nastuta, A.V., Topala, I., Grigoras, C., Pohoata, V., Popa, G., 2011. Stimulation of wound healing by helium atmospheric pressure plasma treatment. Journal of Physics D Applied Physics, 44(10): 105-204.
Rossi, F., Kylian, O., Hasiwa, M., 2006. Decontamination of surfaces by low pressure plasma discharges. Plasma Processes and Polymers, 3(6-7): 431-442.
Srujana, G., Rajender, R.A., Krishna, R.V., Reddy, S.R., 2011. Microbial quality of raw and pasteurized milk samples collected from different places of Warangal District, (A.P.) India. International Journal of Pharma and Bio Sciences, 2(2): 139-143.
Yu, Q.S., Huang, C., Hsieh, F.H., Huff, H., Duan, Y., 2007. Bacterial inactivation using a low-temperature atmospheric plasma brush sustained with argon gas. Journal of Biomedical Material Research Part B: Applied Biomaterials, 80(1): 211-219.